الفهرس 
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Abstract
This study was carried out at Genetics Department, Faculty of Agriculture, Zagazig University. The field investigation were carried out in the experimental Farm at Giza Research Station , El-Nubaria Research Station ,Egypt and AGERI Lab., during the three successive faba bean growing seasons of 2016/2017, 2017/2018 and 2018 /2019. The main objective of this study included the induction of new promising faba bean genotypes that are able to produce high yield and resistance to chocolate spot disease. The current study was performed to identify promising resistant and high-yielding progenies, to study the mode of inheritance for chocolate spot resistance and yield traits using half diallel mating design, and to investigate the association between seed yield and its attributes traits under conditions of chocolate spot disease. Two resistant (Nubaria-1and Sakha-1) and two susceptible (Trible-White and Camolina) parents previously characterized were used to generate six F1 hybrids which were selfed to produce F2 progenies. The parents and their F1 and F2 were evaluated at hot-spot location for chocolate spot disease. Were hybridized to obtain the genotypes resistant to the chocolate spot disease using agro morphological and molecular characterization for selecting promising crosses and the performance under the infection of chocolate spot. Genetic parameters revealed by RAPD and ISSR markers assessed the genetic variation of genotypes with their generations. Crosses 1 (P1 “Nubaria-1” x P2 “Sakha-1”), 2 (P1 “Nubaria-1” x P3 “T.W”), and 3 (P1 “Nubaria-1” x P4 “Camolina”) revealed high resistance to disease with high yield. Markers patterned specific loci of resistant parents at a length of 360, 470, 450, 660, and 140bp in RAPD and 1100, 810, 650, 700, 480bp in ISSR. Inter-varietal hybridization between the resistant and susceptible genotypes is considered one of the most promising methods to obtain germplasm with resistance and high yield. Key words: Vicia faba, chocolate spot, hybridization, ISSR, resistance genetic, plant breeding, RAPD, yield components.