الفهرس 
Title : Studying the relation between the peptide chain release factor methyltransferase PrmC and the pathogenicity of acinetobacter baumannii
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Abstract
Acinetobacter baumannii is a multidrug resistant pathogen that is difficult to treat in both community and hospital-acquired infections because of its remarkable antimicrobial resistance to nearly all available drugs. Consequently, the discovery of novel potential targets for development of new therapeutics is urgently needed. One approach is to determine gene product(s) that play an essential role in the normal physiology and pathogenicity of this organism and target these products with non-conventional therapeutics. PrmC (Peptide chain release factor methyltransferase) methylate the glutamine residue of a conserved GGQ motif of the peptide chain release factors (RFs). It is involved in the regulation of virulence factor production, in Escherichia coli and Chlamydia trachomatis. In Pseudomonas aeruginosa, PrmC was proved to be critical for pyocyanin production. Beeacause of the lack of information about the exact role of the PrmC protein in the physiology and the pathogenesis of A. baumannii, in this work, attention was drawn to further investigate the role of this protein as a potential novel anti-virulence target. Bioinformatics analyses of the A. baumannii PrmC revealed that its gene is highly conserved among A. baumannii strains. Several trials of inactivation of the prmC gene through targeted gene knock-out failed to yield a viable product, sugessting an essential role played by the gene product