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العنوان
Isolation and characterization of salt-induced genes from the halophytic plant cakile maritima /
الناشر
Marwa Hanafy Ahmed Osman ,
المؤلف
Marwa Hanafy Ahmed Osman
هيئة الاعداد
باحث / Marwa Hanafy Ahmed Osman
مشرف / Ebtissam Hussein Aly Hussein
مشرف / Salah Eldin Sayed Mohamed Elassal
مشرف / Mohamed Tawfik Saleh
تاريخ النشر
2018
عدد الصفحات
208 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
الزراعية والعلوم البيولوجية (المتنوعة)
تاريخ الإجازة
7/3/2018
مكان الإجازة
جامعة القاهرة - كلية الزراعة - Genetics
الفهرس
Only 14 pages are availabe for public view

from 268

from 268

Abstract

The halophytic plant (Cakile maritima) possess many unique genetic characteristics contributing to plant{u2019}s ability to withstand and tolerate salinity conditions. It is a member of the Brassicaceae family, with a small genome size (720 Mb) and a short life cycle, making it of value for conducting genetic studies to understand molecular bases of salinity. In the present study, C. maritima seedlings were exposed to different salt treatments for different exposure time to identify new salinity-responsive genes. One group was directly treated with different salinity levels (0, 100, 200, 300 and 400 mM NaCl) for 4, 8, and 12 hrs.; while the second group was subjected to a gradual increase of salt concentration. Some of the transcripts that were differentially expressed in response to the salinity stress were isolated using the differential-display reverse transcription-PCR (DDRT-PCR) technique. Fourteen re-amplified fragments were selected, and were bioinformatically analyzed and annotated