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Abstract
The aim of this study was to isolate, cultivate and identify of Salmonella recovered from slaughtered cows. In the present study standard ISO 6579 method was used to investigate the presence of salmonellae in slaughtered cattle. Salmonellae were detected in 28% gall bladder samples, 24 % duodenum samples, 10% hide swabs,10% liver samples, 8% Pre-femoral (pre-crural) lymph node swabs, 6% Pre-scapular lymph node swabs and 0% raw muscle meat samples. Salmonella isolates were identified as salmonella muenster (17), S. Typhimurium (15), S. Kentucky (4), S. Anatum (3), S. Nyborg (3) and S. Livingstone (1). All isolates were sensitive to azithromycin, Most isolates were sensitive to ceftriaxone (77%), sulfa-trimethoprim and cefotaxime (67% each), cephalexin 63% and cefoperazone 58%. On the other hand, all isolates were resistant to Rifampin and clindamycin. Most Salmonella isolates were resistant to amoxicillin (81%) and cefepime (72%). Confirmation of 43 recovered salmonella serovars by Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) which used as a reliable fast and economic tool for the identification of Gram-negative bacteria especially salmonella which could be used as an alternative diagnostic tool for routine identification and differentiation of clinical isolates