الفهرس | Only 14 pages are availabe for public view |
Abstract Haworthia limifolia Marloth, Gasteria carinata (Mill) Duval and Gasteria minima Poelln. are small succulent plants which belong to family Asphodelaceae according to APG IV classification system. The DNA fingerprinting using RAPD-PCR analysis was made using 10 primers and it revealed that primers OPB-07 and OPB-01, recording high percentage of polymorphism, can be used to differentiate between the examined species. Meanwhile, primers OPB-01, OPB-04, OPB-07 and OPB-08, generating fragments with wide molecular size, can be used for their identification. Macromorphological and micromorphological botanical features of the leaves and stems of the three species under investigation were illustrated using the entire specimens, transverse sections and powdered plant. The preliminary phytochemical screening of their aerial parts revealed the presence of carbohydrates and/or glycosides, condensed tannins, free and combined flavonoids, sterols and/or triterpenes, and free and combined anthraquinones and the absence of essential oil, hydrolysable tannins, saponins, alkaloids and/or nitrogenous bases, cardiac glycosides and oxidase enzyme. Using LC-MS/MS analysis, several compounds belonging to different classes as phenolic acids, flavonoids, anthraquinones and chromones were identified in the investigated species |