الفهرس 
Title : T-cell Polarization in Breast Cancer: A potential Role of Syndecan-1
ContantsOnly 14 pages are availabe for public view
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Abstract
This study aimed to identify the immunomodulatory role of tumor Syndecan-1 (Sdc-1) in the polarization of T helper (Th) subsets isolated from the tumor microenvironment of inflammatory breast cancer (IBC) and non-IBC patients. Two models of co-cultures (direct & indirect) were established with Sdc-1-silenced SUM-149 cells and axillary Th cells of IBC and non-IBC patients. Employing flow cytometry, the frequencies of Th₁, Th₂, Th₁₇, and Treg subsets for basal and cocultured cells were assessed. The data revealed a lower basal frequency of Th₁and Th₂ subsets in IBC than non-IBC. Sdc-1-silenced SUM-149 cells significantly enhanced the polarization of Th₁₇ and Treg subsets of non-IBC under both direct and indirect conditions. Interestingly, qPCR showed a negative correlation between Sdc- 1 and each of IL-4, IL-17, and Foxp3 mRNA expression in carcinoma tissues of IBC that was reversed in non-IBC. Mechanistically, Sdc-1 knockdown in SUM-149 cells promoted Th₁₇ cell expansion via up-regulation of IL-23 and the Notch ligand DLL4. Overall, this study suggests an immunoregulatory role of tumor Sdc-1 expression in Th cell polarization that may have therapeutic implications for breast cancer