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Abstract
MicroRNA-26a (miRNA-26a) is known as one of tumor suppressors that prevent propagation and invasion of hepatocellular carcinoma (HCC) cells based on its ability to target various oncogenic genes. Dysregulation of miRNA-26a and its downregulation have been observed during initiation and progression of HCC. Thereby, this study was designed to assess the potential protective efficacy of miRNA-26a administration ondiethyl nitrosamine (DEN)-induced HCC mouse model. After 8, 12, 16, and 20 weeks of follow up, blood samples were collected to determine frequency of regulatory T (Treg) cells by flow cytometry.Concentrations of tumor markers including AFP (alpha fetoprotein) and DCP (des-gamma carboxyprothrombin) were estimated by Enzyme linked immunosorbent assy ( ELISA( . Levels of T helper (Th1)-related cytokines [interleukin (IL), IL-2 and tumor necrosis factor-alpha (TNF-Ü)] and Th2- related cytokines (IL-10) as well as vascular endothelial growth factor (VEGF) were also measured by ELISA. Relative expression levels of survivin and caspase-3 were investigated by real time polymerase chain reaction (PCR). Concentrations of tumor markers and frequency of Treg cells that were elevated during HCC induction showed significant lower values following miRNA- 26a administration