الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Sealants are proven efficacious in carious lesion prevention and arrest on the occlusal surfaces of teeth. The incorporation of microcapsules with anticaries properties into the pit and fissure sealant material to provide a sustained ion release can add beneficial feature to the conventional fluoride releasing sealants. This in vitro study was conducted to assess the effect of BioCoat Bioactive pit and fissure resin-based sealant with incorporated microcapsules on inhibition of enamel demineralization in permanent molars in comparison Clinpro fluoride releasing resin-based sealant and Helioseal Clear non-fluoride releasing resin-based sealant.
Methods: Thirty nine extracted human third caries-free molars were selected. Cavity preparations were made on the buccal surface of the specimens and randomization took place in which the thirty nine molars were divided into three groups, 13 specimens for each group. group 1 (test group) received BioCoat Bioactive resin pit and fissure sealant with microcapsules, group 2 (test group) received Clinpro resin fluoride releasing sealant and group 3 (positive control group) received Helioseal Clear resin non-fluoride releasing sealant. Acid-resistant varnish covered the specimens excluding a 1millimeter band of bare enamel for in-vitro enamel caries formation. Thermocycling of teeth took place, then the teeth were exposed to an artificial caries medium for six weeks to produce caries-like lesions in the bare enamel area. Transverse sections of each tooth were inspected using polarized light microscopy and analyzed using Image J software to determine the lesion depth and assessed histologically to evaluate the changes in enamel characteristics and compare the extent of carious lesion among the three study groups.
Results: The two test groups BioCoat and Clinpro were significantly lower P<0.0001 in the mean lesion depth when compared to the positive control group Helioseal Clear. group 1 BioCoat Bioactive sealant exhibited the least mean lesion depth of 53.58 ± 25.74 while group 3 Helioseal Clear sealant exhibited the greatest mean lesion depth of 163.49 ± 55.01. Both test groups BioCoat and Clinpro had a remarkable reduction percentage in mean lesion depth of 58.06% ± 34.13 and 54.83% ± 20.67 respectively when compared to Helioseal Clear group. Histologically, group 1 BioCoat Bioactive sealant showed the most prominent reduction in the extent of the carious lesion with a thick remineralization band.
Conclusion: Both BioCoat Bioactive resin sealant and Clinpro fluoride releasing resin sealant have a significant inhibiting effect on enamel demineralization in permanent molars in comparison to Helioseal Clear non-fluoride releasing resin sealant. BioCoat Bioactive sealant showed more remineralizing capability due to the incorporation of bioactive microcapsules filled with anticarious fluoride, calcium and phosphate ions.