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Abstract Introduction health care services provided at Neonatal Intensive Care Units(NICU) are to respond to client{u2019}s needs and expectations and be suitable to health care provider{u2019}s situation in order to maintain appropriate level that will be reflected on health of neonates. Aim of the study Assessment of health care services provided to neonates in NICU to improve them with subsequent reduction of neonatal morbidity and mortality. Subjects and Methods A cross sectional study conducted at NICU in BeniSuif University Hospital between June 2013 and July 2014. The study included all neonates admitted to the NICU during the study period (146) and all NICU health care providers (13 physicians and 9nurses). It included assessment of quality of NICU service structure, care, infection control practices, satisfaction of care takersand health care providers. Results the most common causes of admission were respiratory distress (RD), jaundice, sepsis and heart failure (H.F.) (73.3%, 11.6%, 11% and 5.5% respectively).Age on admission ranged between 1 and 24 days.Only two neonates1.37% were exclusively breastfeeding while the others received formula or mixed feeding (85.6% and 13.01% respectively). The most common maternal complicationswere Diabetes Mellitus(D.M.)and allergy. Guidelines and protocols of Ministry of Health and Population (MOHP), 2010 are present in NICU. One third of place requirements and 10.5% of equipmentswere not available. The nurse to incubator ratio was 1:3during morning and 1:6 during night shifts. Routine investigations and therapy were carried out for all cases. Phototherapy was the primary management in all cases of neonatal jaundice supported by exchange transfusion only in 2 cases. The most frequent diagnoses at discharge were respiratory distress, pneumonia, bronchiolitis, neonatal sepsis, neonatal jaundice and death(20.5%, 13%, 9.6 %, 7.5%, 6.8% and 25.3%) and 25.4% died.Nurses were less satisfied than physicians |