الفهرس 
Comparison of the healing potential of propolis and Bee venom on surgically induced wound in rat{u2019}s buccal mucosa
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Abstract
In this study 45 adult male albino rats of average weight 150-200 grams divided into 3 groups (n=15 per group) were used (C: control, P: propolis and BV: bee venom). All rats were maintained on basic diet of regular rat chow and distilled water adlibitum. They were housed under controlled environment (temperature 25±2{u00B0} and 12 hr. dark / light cycles) at the animal house of faculty of Medicine, Cairo university, Egypt. All animals were anesthetized with a mixture of 13 mg/kg of xylazine (2%) and 33 mg/kg of ketamine via in»tramuscular injection. After anesthesia, the cheek were pulled and a wound were induced opposite to mid buccal premolar area using sterile punch biopsy instrument (3.5mm diameter). For intra oral topical application, a syringe with an attached sterile metal tube was loaded with 0.5 ml of propolis, bee venom or saline solution and applied onto the wound lesion immediately after wound induction. 5 rats from each group were sacrificed after 1, 3 and 7 days. The buccal mucosa containing the induced wound was dissected. Each wound was excised using a (5.5 mm diameter) punch biopsy instrument. Then fixed in 4% buffered formalin for 24 hours, dehydrated in ascending grades of ethyl alcohol, cleared in xylene and embedded in paraffin to get 4-5 microns thick sections which were prepared for histological examination using hematoxylin and eosin stain, masson trichrome stain and Immunohistochemical examination using PCNA