الفهرس 
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Abstract
As one of the world’s dangerous diseases, cancer is a serious threat to human health. Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer. It is an end-stage liver disease that develops as a complication of fibrosis and cirrhosis. Liver cancer is a major public health concern. HCC is characterized by rapid development, a high degree of molecular and histological heterogeneity, and a bad prognosis. The tumor suppressor gene P53 contributes to the emergence of HCC due to an imbalance between excessive cell growth and apoptosis.
The current study aimed to:
- Induction of hepatoma model by DENA and CCl4 with focusing on the induced changes in the liver and bone marrow.
- Isolation of AD-MSCs from male rats and characterization of them.
- Investigate the action of AD-MSCs on the liver and BM of DENA/CCL4-induced HCC model.
- Highlight the modifications of F.(AD-MSCs) on human hepatoma cell lines (HepG2 and Huh7).
Materials and Methods:
Experiment 1 (in vivo)
in this study, adult female albino rats (n = 45) were purchased from the Assiut University Experimental Animal Breeding Unit. Animals were randomized and subdivided into three groups (n = 15) in each: G1: (15 rats) served as a control group. G2 :(15 rats) served as the HCC model group. Each rat was injected with a single dose of DENA (100 mg/kg (i.p.), and after one-week, CCl4 was injected in a dose of 0.2 ml/kg b.w., i.p. diluted in corn oil to a final amount of 10 ml (0.6 percent, v/v) injected twice a week for two weeks. G3: (15 rats) served as a therapeutic group; this group treated as G2 and then was injected after a tumor appeared (8 weeks), with (0.65 x 106) AD-MSCs in 0.5 ml of PBS, then sacrificed 14 days later. After the expiration of the treatment, all rats were anesthetized and dissected for the collection of blood samples, liver, and BM for biochemical analysis and histological examination. Experiment 2 (in vitro)
Two HCC cell lines (HepG2 and Huh7) were purchased from Nawa Scientific Research. subdivided into three groups. G1: served as a control group for HepG2 or Huh7 cell lines (2x103cells/well) with 30 µL medium and serum. G (2): HepG2 or Huh7 cell lines (2x103cells/well) were treated after 1 hour with fragmented frozen AD-MSCs (6x104 cells) at a concentration of 1:15 µL. G (3): HepG2 or Huh7 cell lines (2x103cells/well) were treated with fragmented frozen AD-MSCs (6x104cells) at a concentration of 1:30 µL. The viability and apoptosis of the HCC cell line were evaluated.