الفهرس 
Hypertrophic scarsOnly 14 pages are availabe for public view
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Abstract
Abstract
Background: Hypertrophic scar (HTS) is a dermal form of fibro-proliferative disease that develops after deep burns, skin injuries, and surgical wounds. HTS is defined as a visible, elevated scar that does not spread into surrounding tissues. MicroRNAs (miRNAs) represent a class of small, 18- to 28-nucleotide-long, noncoding RNA molecules. They have a major role in the post-transcriptional regulation of protein expression, and their involvement was demonstrated in normal and in pathological cellular processes. Several miRNAs are involved in the regulation of key processes that contribute to hypertrophic scarring.
Aim of the work: The aim of our study is to investigate the role of miRNA (181a), (214), (199a) and (363) in the pathogenesis of hypertrophic scar by measuring its levels in skin biopsy and serum of patients compared with controls.
Subjects and Methods: This study was a case control cross- section study, conducted in Dermatology department of Fayoum university hospitals, between March 2020 and October 2020. The patients group included 20 patients (18 females and 2 males). Patients age were ranged from 16 to 48 years, Mean ±SE (24.80±2.20) and 20 control (17 females and 3 males) their ages ranged from 17 to 48 years, mean ±SE (31.05±2.31). Serum and tissue micro-RNA (181a), (214), (199a) and (363) where extracted for both groups and amplified using real time PCR.
Results: In our study there was highly statistically significant difference between patient and control groups as regard miR-181a , miR -214, miR-363 and miR -199a levels in serum and tissue with p=0.0001.where miR-181a and miR -214 levels in serum and tissue were much higher in patient group than control group. This indicate
the possible role of miR-181a and miR-214 in the pathogenesis of hypertrophic scar. While miR-363 and miR -199a levels in serum and tissue were decreased in patient group compared with their levels in control group. Also there was positive correlation between miR-181a and miR-214 in serum and tissue among Hypertrophic scar patients.
Conclusion: We found that miR-181a and miR-214 were upregulated in serum and tissue of patients compared with controls suggesting their role in pathogenesis of hypertrophic scars. While miR-199a and miR-363 were downregulated.
Key words: hypertrophic scars, micro-RNA (181a, 214, 199a, 363).