الفهرس 
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Abstract
This study was carried out in a broiler farm under the supervision of Animal Production Department, Faculty of Agriculture, Tanta University during the period from October to November 2019 to investigate the effects of supplementation of grape seeds at different levels on growth performance, blood biochemical profiles and immune response of broiler chicks. Three hundred one-dayold unsexed Cobb broilers chicks (1: 35 old day) were used in this experiment. Birds were wing-banded, individually weighed to the nearest gram, and randomly distributed into four equal experimental groups; with three replicates of 25 chicks each. The average initial body weights of the treatment groups were nearly similar with no observed significant differences. The chicks were offered two cornsoybean meal basal mash diets that were formulated to meet or exceed the Nutritional Requirement of growing chicks as recommended by the National Research Council (NRC 1994). The experimental designed was included: Control Basal diet without any addition T1 (0.5 GS) Basal diet plus 0.5% grape seeds T2 (0.75 GS) Basal diet plus 0.75% grape seeds T3 (1.0 GS) Basal diet plus 1.0% grape seeds All birds were subjected to the same managerial, hygienic and environmental conditions. The birds were grown in floor litter condition and subjected to 24 hrs, which extended to 5 weeks of age. The house temperature was kept at about 33ºC during the first 3 days, 32ºC during next 4 days and thereafter, gradually decreased by 2-3ºC weekly down to 22-24ºC. Mash feed form and water were available ad labium throughout the experimental period. Individual live body weight and feed intake were recorded weekly during the experiment. Weight gain and feed conversion ratio were calculated on a group. At the end of the feeding trial, all birds were fasted for 12 hrs prior to final sampling. Three birds from each replicate were randomly selected weighed, slaughtered by slitting the jugular vein of the birds in the morning, then after slaughter and complete bleeding scalded and defeathered. Carcasses chicks were eviscerated manually and weighed. Liver, spleen, bursa, heart and thymus were removed carefully and weighed. All organs weights were expressed as percentage of body weight and subjected to arcsine transformation. Three blood samples were collected from three birds in each group during slaughtering, one with EDTA while the other without; tubes in EDTA-containing for determination of the biochemical parameters. Chicks of plasma or serum were obtained immediately by centrifugation the blood for 10 min. at 3000 rpm and frozen rapidly in ependorf tubes until the time of analysis. Total protein, liver function enzymes, lipid profile and antioxidant status were measured. The results indicated that:- 1- Broilers in all treatments, which were dieted with grape seeds had a significant effect on body weight as comparing to control group. The highest value was in the birds fed on 0.75 % grape seeds followed by 1.0 % grape seeds, then the birds fed on 0.5% grape seeds compared to control ones. 2- There are a significant effect of grape seeds on feed consumption and feed conversion ratio. Birds fed 0.75% grape seeds had the best value, then birds fed 1.0 % grape seeds followed by 0. 5% grape seeds group. 3- Broilers dietary with grape seeds had no significant effect on mortality rate as compared with the control. 4- There is had no significant effect of broilers diet with grape seeds on relative weight of liver, spleen and heart. On the other hand there were significant effects of broiler diet with grape seeds on carcass percentage and relative weights of bursa and thymus. 5- Grape seeds had no significant effect on the concentration of AST and ALT. On the other hand an enhancement was found in values of total protein, globulin, albumin, HDL , LDL, total lipids, triglyceride, total cholesterol, total antioxidant capacity, MDA and SOD.