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العنوان
Study of keratinase production and keratin degradation by environmental microbial straine isolated from natural keratinaceous materials (feather) in Egypt /
الناشر
Eltahir Ahmed Sabiel Ahmed ,
المؤلف
Eltahir Ahmed Sabiel Ahmed
هيئة الاعداد
باحث / Eltahir Ahmed Sabiel Ahmed
مشرف / Manal Mohamed Maher
مشرف / Ahmed Sherif Attia
مشرف / Elshahat Hassan Abdallatif Nashy
مشرف / Azza M.M. Abdel Fattah
تاريخ النشر
2016
عدد الصفحات
171 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
صيدلة
تاريخ الإجازة
9/3/2016
مكان الإجازة
جامعة القاهرة - كلية الصيدلة - Microbiology and Immunology
الفهرس
Only 14 pages are availabe for public view

from 203

from 203

Abstract

The present work has started by a microbiological survey for microbial production of keratinase enzyme through transformation of keratin (substrate of concern) into useful hydrolysates. The competitive battle for the selection of the best keratin - loving amongst the stating 43 and the semi - final 14 microbes ended by cyberlindnera fabianii NRC3 aza. Time course of this microbe for keratinase production by submerged fermentation (SmF), started from the first day and reached its maximum level (1706.67U / ml, 183.51 U / mg protein) on the third day. Complete solubilization of the soild feather occurred by day 4 of cultivation when the culture solution appeared as a suspension. Nomenclature as cyberlindnera fabianii had been applied to the isolate and was suffixed by NRC3 aza. The isolate was suggested to a novel species of candida. Electrophoresis was carried out for the batch precipitated keratinase enzyme partial pure fraction with 100% ammonium sulfate