الفهرس 
Title : Impact of microRNA-375 and its target gene polymorphism SMAD-7 on susceptibility of colorectal cancer
AbstractOnly 14 pages are availabe for public view
 |  | from | 127 |  |  |
| | | from | 127 | | |
Abstract
Background and aim: Colorectal cancer (CRC) is a major cause of morbidity and mortality throughout the world. It accounts for over 9% of all cancer incidence. It is the third most common cancer worldwide and the fourth most common cause of death. We aim to study the expression of microRNA-375 in serum and rs4939827 SMAD-7 polymorphisms in DNA extracted from blood in colorectal cancer patients and correlate with control subjects. Subject and methods: Blood samples were taken from 86 patients with colorectal cancer (different grades) and 36 normal persons to serve as a control group. The following were done: history taking, general examination, CBC, liver function test, kidney function tests and CEA. Detection of Single nucleotide polymorphism (SNP) of SMAD7 (rs4939827) in DNA extracted from blood by real time PCR. microRNA-375 quantitation in serum by real time PCR. Results: We found that the wild type (CC) genotype was higher in controls 36.1% than in cases 15.1%(p=0.01). On the other hand there is no statistical significance difference as regards to mutant and heterozygotes genotypes. There is higher percentage of TT genotype in patients than in controls (33.7% vs 22.2%, p=0.3). Although there is higher percentage of CT genotype in patients than in controls (51.1% vs 41.7%, p=0.4). There is statistically significance difference between study groups (case and controls) as regards to distribution of different gene alleles with higher percentage of T-alleles in cases than controls (59.3% vs 43.1%, p=0.02) and C-alleles in controls than patients (56.9% vs 40.6%, p=0.02). There is statistically significance difference with p-value <0.001 between study groups as regards to gene expression of mi-RNA 375 with low mean among cases