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Abstract
Microsporidial infection presents a real risk on the stability and progress of the general condition of cancer patients. Detection of non routinely tested microsporidial and coccidial infections among cancer patients was aimed by this study. A total of 200 individuals were included; 100 diarrheic cancer patients (study group) and 100 diarrheic non-cancer patients (control group). Using Ryan blue Modified Trichrome stain, microsporidia infection was detected in 19% of cancer patients and 4% of the control group (p-value 0.001).Using Modified Kinyoun Acid Fast Stain, Cryptosporidium spp. and C.cayetanensis were shown to affect 9% and 2% of study group respectively and 3% and 1% of the control group respectively. Total parasitic infection detected was 64% among cancer patients which was nearly double that in non-cancer patients (27%) and was highly significant (p-value 0.0000). Blastocystis infection was markedly high; 31% in study and 18% in control group (p-value 0.0325). Mixed parasitic infections were detected in 10% of the study group and 2% in the control group (p-value 0.01). Further confirmation of microsporidial infection was done using Transmission Electron Microscopy (TEM) for 18 samples; 13 positive and 5 negative using MT stain. Also, PCR confirmation of 21 samples was done; 15 positive and 6 negative using MT stain. Among the tested samples, E.bieneusi infection was confirmed using both methods. No Encephalitozoon spp. infection was detected by PCR. However, TEM study confirmed Encephalitozoon spp. criteria including E.intestnalis