الفهرس | Only 14 pages are availabe for public view |
Abstract A total of 500 samples of various origins; 400 fishes Tilapia niloticus, Mugil cephalus, catfish & carp fishes), 50 pond water & 50 human (skin swab fisher men) samples were collected from (private and governmental farms) from different localities within Suez Canal area for phenotypic and genotypic identification of some potential toxigenic fungi, as well as molecular characterization of aflatoxigenic fungi in addition to aflatoxin analysis using ELISA technique. The findings revealed that the frequency distribution of total fungal isolates were 83 % from fishes, 13 % from pond water and 4 % from human. Surface samples showed the highest percentage of isolates followed by gills and internal organs., four fungal genera were recovered. Aspergillus spp. (71%) followed by Penicillium spp. (18%) with low occurance of Mucor spp. (7%) and Alternaria spp. (4%). all A.flavus tested isolates were positive by 100% to omt-1 & ver-1 genes, while A.parasiticus isolates was positive by 84% both aflatoxigenic genes (omt-1 & ver-1).most of samples contain aflatoxin with variable concentrations below the permissible limits. |