الفهرس | Only 14 pages are availabe for public view |
Abstract Corynebacterium pseudotuberculosis (C..pseudotuberculosis) is the causative agent of caseous lymphadenitis (CLA)which is a chronic, suppurative disease with a worldwide distribution. It affects small and large ruminant, horses and pigs. In this study we detect the prevalence of CLA in camels, the virulence phosopholipase D (PLD) gene and other virulence genes in the identified organism as PLD gene of C. pseudotubrculosis through use of polymerase chain reaction (PCR). A total of 792 carcasses of camel were examined in three abattoirs in Egypt, among this a number of 92 (11.61%) carcasses were affected with caseous lymphadenitis. The number of carcasses affected with visceral form; 69 (75%), were significantly higher than those affected in the peripheral lymph node; 23 (25%). Camels less than seven years old appear more susceptible for infection by visceral form than older one. The prevalence of C. pseudotuberculosis was 18.5%. based on the nitrate reduction test, the target C. pseudotubercullosis isolates was belong to biovar II. C. pseudotuberculosis isolates recovered in this study were subjected to PCR amplification using pld gene-specific primers. The agarose gel electrophoresis showed an amplification of size area of 924bp in all tested isolates. In this study, the antimicrobial resistance profiles of C. pseudotuberculosis to antimicrobial agents varies among identified bacterial isolates. However, the antibacterial sensitivity revealed that the most effective antibiotic to the organisms was Norfloxacin (100%). |