الفهرس 
Evaluation of Commercial ELISA for Helicobacter pylori (Ag) in Stool Comparing To PCR Assay
AbstractOnly 14 pages are availabe for public view
 |  | from | 142 |  |  |
| | | from | 142 | | |
Abstract
Helicobacter pylori (H. pylori)is one of the most common infectious agents in the world which causes a different forms and degree of gastrointestinal diseases including gastritis, peptic ulcer and gastric carcinoma. Laboratory diagnosis of H. pylori infection is made by invasive and non-invasive methods. Invasive methods require endoscopy which is uncomfortable and unacceptable by the most patients. Therefore, non-invasive methods particularly serological tests are easier and comfortable for patients. The objective of this study was to comparatively evaluate noninvasive direct ELISA which detect Ag of H.pyloriand PCRin diagnosis of infection withH. pyloriinhuman stool and faecesof dogs and cats.Two hundred stool samples from humans and eighty-eight fecal samples from dogs and cats were collected with gastric problems.The examination of samples by ELISA of the 200 human stool samples revealed 27 positive from 200 samples with a percentage of 13.5 % and in feces of dogs and cats revealed 18 positive from 88 samples with a percentage of 20.5 % . also, In an examination, by using semi-nested PCR assay the result was positive in 36 of 200 human specimens with a percentage of 18 %; while 20 of 88 dog’s and cat’s specimens with a percentage of 22.7 %. The sensitivity and specificity of ELISA compared to a standard test by PCR reveal 64.86% and 98.16%respectively depending on the data of human samples, where the animal’s data reveals sensitivity 85.71% and specificity 100% respectively