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Abstract Ag NPs have wide medical and industrial applications and they are significantly released into aquatic environments. The current work aimed to evaluate (for the first time) the adsorption potential of OP and BP used, in water treatment, to reduce the toxicity of Ag NPs in (Oreochromis niloticus). Fish were divided into 4 groups: 1- Control group (dechlorinated tap water), 2- Ag NPs (4 mg/L) exposed group, 3- Ag NPs (4 mg/L) + OP (40 mg/L) group, and 4- Ag NPs (4 mg/L) + BP (40 mg/L) group for 24 h., 48 h. and 96 h. The adsorptive potential of Ag NPs on both peels was confirmed by the SEM, Energy Dispersive X-Ray Spectroscopy and map analyses. Fish exposed to Ag NPs showed a significant decrease in RBCs, Hb, Hct and MCH besides elevated MCV after 48 & 96 h. There was significant gradual elevation in plasma glucose, total proteins, globulin, AST, ALT, ALP, creatinine and uric acid after Ag NPs exposure. While albumin and total lipid have significantly decreased. Antioxidant biomarkers in gills and liver tissues after Ag NPs exposure revealed severe oxidative damages (maximally after 96 h.) as shown by the increase in CAT, SOD, Gpx & TBARS and decrease in GSH. Ag NPs accumulation revealed marked increases as follows: liver > kidney > gills > skin > muscles. Different histopathological alterations caused by Ag NPs have been observed in the gills, liver and kidneys. All studied parameters are restored more or less values near to the control groups after treatment with OP and BP. Both peels showed good adsorptive ability toward Ag NPs especially BP, based on the high Ag concentrations in BP, throughout the experiment and the improved status of fish |