الفهرس 
Immunobiology of ITPOnly 14 pages are availabe for public view
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Abstract
Immune thrombocytopenia (ITP) is an autoimmune condition characterized by increased platelet destruction and suppression of production resulting in an isolated thrombocytopenia. The criteria for a diagnosis of ITP is, an otherwise, isolated unexplained low platelet count of < 100 × 10⁹/L. ITP patients, compared with individuals without ITP from the general population, have 1.5‐fold and 1.7‐fold elevated risks of arterial cardiovascular complication and venous thromboembolism due to the presence of large and immature platelets, thrombosis risk factors, and increased levels of antiphospholipid antibodies.
Carotid intima-media thickness (CIMT) is a non-invasive method to detect early subclinical atherosclerosis and it correlates well with overall vascular injury and extend and severity of coronary artery disease. CIMT ultrasonography may represent an accessible and reliable method to detect subclinical atherosclerosis so it predicts future cardiovascular and ischemic stroke incidence.
MiR-31 is one of the family of MicroRNAs (miRNAs) that are conserved small non-coding regulatory RNAs that mediate translational repression. MiR-31 was found to be involved in diverse biological processes including fertility, embryonic development, bone formation, and myogenesis. It has also been shown to be mis-regulated in a number of diseases, including cancer and autoimmune diseases, such as psoriasis and SLE.
We aimed to evaluate the possible correlation between miR-31 and CIMT in chronic adult ITP patients to early detect early atherosclerosis
In our study, we included 84 patients, 42 of them diagnosed as primary immune thrombocytopenia (ITP), we selected them from the outpatients and inpatients of Hematology department of Menoufia University Hospitals during the period from first February 2020 to the end of October 2022. We compared the selected ITP patients to controls of 42 normal persons matched by age and gender.
10 ml blood sample using EDTA as an anticoagulant was collected from each participant after fasting 12 hours from peripheral veins. After that, serum centrifugation at 1800g under 4 °C for 15 min was done and biochemical tests were performed.