الفهرس يوجد فقط 14 صفحة متاحة للعرض العام
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المستخلص
Hepatocellular carcinoma (HCC) is the most common histological type of primary liver cancer, In Egypt, HCC account for about 4.7% of chronic liver disease. Approximately 85% of HCC cases are presented at late stage. MicroRNAs (miRNAs) are a non-coding family of genes involved in post-transcriptional gene regulation. These transcripts are associated with cell proliferation, cell differentiation, cell death and carcinogenesis. The expression of 8 miRNAs in serum and tissue of 34 primary HCC patients and serum of 52 patients with hepatitis C virus as well as 50 healthy controls was analyzed using qRT-PCR real time technique. Also the correlation between the studied miRNAs was analyzed versus each of the clinico-pathological factors collected in serum of primary HCC patients using T-test. Significant deregulation in serum level of miRNAs was detected in 7out of 8 miRNAs studied where 6 were up regulated (miR-23b, miR-193b, miR-215, miR-125b, mir-122 and miR-130a) and 1 was down regulated (miR-126) as compared to healthy normal controls. No concordance was reported in miRNAs expressed in either serum or tissue samples. Finally, significance association was observed in the expression level of miR- 126 with cirrhotic status of liver. In conclusion, the current study showed that among the eight examined miRNAs, miR-126, miR-193, miR122, miR-23b, miR-130a and miR-125b may be used as potential biomarkers specific to diagnose primary HCC. Finally, miR-126 may play important role in cirrhotic HCC patients