الفهرس 
HEPATOCELLULAR CARCINOMAOnly 14 pages are availabe for public view
 |  | from | 238 |  |  |
| | | from | 238 | | |
Abstract
Background : the detection and analysis of circulating nucleic acids in blood may aid not only in disease diagnosis but also in guiding molecular targeted therapy, monitoring response to such or other treatments, as well as identifying mutations associated with drug resistance. Furthermore, unlike a tumor biopsy, circulating nucleic acids may provide useful information regarding tumor heterogeneity .
Objective compare ctDNA by targeting the hotspot mutation gene locus TP53 rs28934571 (c.747G>T) in normal persons, chronic liver disease patients, early and late HCC cases to evaluate its diagnostic value and analyse ctDNA
Patients and Methods: It was performed on 100 adult subjects. Patients were recruited from October 2020 through June 2022 from Ain Shams Centre for Organ Transplantation and Tropical Medicine Department, Ain Shams University Hospitals, including: Eighty (80) patients diagnosed with HCC on top of HCV according to European Association for Study of the Liver (EASL) guidelines. In addition, a non-HCC group was included: ten (10) HCV-positive patients and ten (10) healthy controls were recruited to the study (non-HCC group). HCC clinical stage was determined according to Barcelona Clinic Liver Cancer (BCLC) staging classification and Child-Pugh classification
Results There was no significant statistical difference between the HCC group, healthy control and HCV control group as regards age (p value =.09) , sex (p value =1) and comorbidities. AST was found statistically significant in HCC group in comparison with healthy control group (p value =.018)and there was statistical significant difference between HCC group and (healthy and HCV) control groups as regards albumin(p value =.03) and INR( p value <.001). 40 patients (50 %) were within Milan criteria, 12 patients (15%) were within USCF,28 patients (35%)were beyond all and 39 patients (48.75%) were child A. Mean meld score was 12.45 ± 5.94. As regards the tumor charecterictics in the explant pathology of HCC patients who underwent liver transplantation ,the mean size of the focal lesion was 3.66 ± 2.26 and four patients had vascular invasion in their pathology.
Conclusion: detecting ctDNA in peripheral blood enables
us to track the traces of tumor in high-risk population and obtain genomic profiles of advanced HCC patients in a minimally invasive manner.