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العنوان
Genetic improvement and germplasm conservation of Some Fruit Cultivars /
الناشر
Rehab Nazih Turki Almousa ,
المؤلف
Rehab Nazih Turki Almousa
هيئة الاعداد
باحث / Rehab Nazih Turki Almousa
مشرف / Amina Hamed Gomaa
مشرف / Ramzy George Stino
مشرف / Neveen Abdelfatah Hassan
تاريخ النشر
2014
عدد الصفحات
408 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البساتين
تاريخ الإجازة
7/4/2015
مكان الإجازة
جامعة القاهرة - كلية الزراعة - Pomology
الفهرس
Only 14 pages are availabe for public view

from 432

from 432

Abstract

In this study, different approaches for micropropagation, in vitro conservation and cryopreservation of grape ”Black Matrouh” and guava ”Al-Baladi”, and in vitro mutagenesis for inducing grape plantlets more tolerant to drought were investigated. Grape shoot tip and nodal explants showed the highest survival% on {u00BE}MS medium with 0.5 mgl-1 BAP, while MS medium with 0.5 or 1.0 mgl-1 BAP produced the highest shoot no./shoot tip or nodal explant, respectively. Established explants cultured on {u00BE}MS medium with (0.75BAP+0.50 IBA) mgl-1 or (1.00BAP+0.30IBA) mgl-1 produced the highest shoot no./explant, while established explants cultured on growth regulator-free MS medium showed the highest shoot length. The highest rooting% was recorded on {u00BD}MS medium with 1.0 mgl-1 IBA and the longest roots on {u00BD}MS medium with 0.5 mgl-1 IBA without significant differences compared to {u00BD}MS medium with 0.5 mgl-1 NAA which exhibited the highest roots no./explant. Guava nodal explants cultured on MS medium with 1.0 mgl-1 BAP showed the highest survival% and shoot no./explant. Meanwhile, explants cultured on growth regulator-free MS or WPM medium failed completely to establish. Established explants cultured on MS medium plus 4.5% sucrose and (0.75BAP+40ADS) mgl-1 or (0.75BAP+80ADS) mgl-1 produced the highest shoots no./explant. While, MS medium plus 4.5% sucrose and (0.50BAP+40ADS) mgl-1 showed the tallest shoots