الفهرس 
AbstractOnly 14 pages are availabe for public view
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Abstract
CKD is an ongoing worldwide burden in public health. It affects the quality of life of millions of people, placing CKD as the fastest growing causes of premature death worldwide. The main issues remain the heterogeneity of the disease and the lack of tools for efficient diagnosis and prognosis. miRNAs have been shown to play a key role in different pathological conditions, including CKD, and could serve as biomarkers and/or therapeutic targets. Urinary miRNAs may also serve as non – invasive biomarkers, a tool needed for accurate and fast diagnosis and prognosis of CKD, to replace kidney biopsies
This study aimed to evaluate the levels of circulating total and mRNA 21, mRNA 155 CT, the interest in their physiological functions and their potential utility as biomarkers in chronic kidney disease.
This study was conducted on a total of fifty patients with chronic kidney diseases at Beni-Suef Hospitals and Clinics comparing them with thirty normal volunteers as control group.
A total of 50 cases were included in this study, the cases were divided into three groups according to clinical diagnosis:
Stage 3 group: Fifteen (15) patients with kidney disease stage 3.
Stage 4 group: Fifteen (15) patients with kidney disease stage 4.
On dialysis group: Twenty (20) patients on dialysis.
The main results of the study revealed that:
Comparison between the four groups showed that means of age and weight were statistically significant different between groups where the mean of age and weight in the control group was statistically significantly lower than the other three groups.
This study shows the distribution of males and females in the four groups, where there was no statistically significant difference found by comparison between the four groups as regards gender (p˃0.05).
This study shows the distribution of chronic disease in the groups of stage 3 & 4, and on dialysis group, where there was no statistically significant difference found by comparison between the groups as regards chronic diseases (p˃0.05).
Comparison between the four groups showed that means of GFR and creatinine were statistically significant different between groups with where the mean of GFR was statistically significantly decreasing as the stage increase, while the creatinine was statistically significantly increasing as the stage increase.
Comparison between the four groups showed that means of hemoglobin and albumin were statistically significant different between groups with where the mean of hemoglobin and albumin were statistically significantly decreasing as the stage increased.
Comparison between the four groups showed that means of phosphorus and calcium were statistically significant different between groups with where the mean of calcium was statistically significantly higher in control group than the other three groups, while phosphorus was increasing as the stage increase, but statistically significantly lower in control group than the other three groups.
Comparison between the four groups showed that means of mRNA 21 CT and mRNA 155 CT were statistically significant different between groups with where the mean of mRNA 21 CT and mRNA 155 CT were statistically significantly decreasing as the stage increase.
According to Spearman’s rank correlation coefficient analysis, the Level of mRNA 21 CT was positively correlated with Glomerular filtration rate, Hemoglobin, Albumin, Calcium, and the Level of mRNA 21 CT was negatively correlated with stage, age creatinine, and phosphorus, and no correlation with weight.
According to Spearman’s rank correlation coefficient analysis, the Level of mRNA 155 CT was positively correlated with Glomerular filtration rate, Hemoglobin, Albumin, Calcium, and mRNA 21 CT, and the Level of mRNA 155 CT was negatively correlated with stage, age creatinine, and phosphorus, and no correlation with weight.
Based on our results we recommend for further studies on larger patients and longer period of follow up .