الفهرس 
CoverOnly 14 pages are availabe for public view
 |  | from | 66 |  |  |
| | | from | 66 | | |
Abstract
Methotrexate is a chemotherapy used in treatment of rheumatoid arthritis (RA), and other autoimmune diseases. However, its long-term administration causes testicular adverse effects. Carvacrol is a phytochemical found in aromatic plants of the Oregano family. It possesses anti-inflammatory and anti-oxidant properties and can supress cancer cells growth. The present study was done to estimate the antioxidant effect of carvacrol on MTX-induced testicular damage. Twenty-four adult rats were randomly divided into four groups, six rats each, as follows: Control, Carvacrol (40mg/kg b.w. daily orally), MTX (25 mg/kg b.w single shot I/P) and Carvacrol and MTX. Testicular biomarkers, redox status, Nrf2 gene expression and histopathological examinations were estimated after 15 days. MTX caused changes in testosterone hormone, total and prostatic acid phosphatase enzymes. Moreover, testicular 8-OHdG and MDA were increased while reduced GSH was decreased indicating testicular oxidative damage. Coadministration of carvacrol was able to modulate the adverse effects of MTX. This was observed in the retrieved levels of the mentioned parameters nearly to normal in comparison to the control and MTX groups. In the present study, we state that carvacrol ameliorates MTX-induced testicular damage by protection from oxidative stress, decrease in DNA damage, and enhancement of serum testicular biomarkers.