الفهرس 
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Abstract
Primary immunodeficiency disorders (PIDs) are a diverse class of more than 150 hereditary diseases marked by a lack of function in one or more innate and adaptive immune system components.
Chronic granulomatous disease (CGD) is one of the PIDs caused by defective phagocytic function due to a problem with the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase complex. When phagocytes are activated, NADPH oxidase complex proteins that are both intracellular and membrane-bound work together producing reactive oxygen species (ROS), which are crucial for eliminating invasive bacteria and fungi.
Neutrophils and monocytes’ NADPH oxidase complexes are functionally impaired in CGD due to mutations in the genes encoding for the complex’s subunits. These mutations can be X-linked or autosomal recessive.
B lymphocytes signaling and differentiation are ROS dependent. Therefore, the transformation of B lymphocytes from naive into memory B cells can be impacted by the NADPH oxidase subunits loss in CGD patients.
This study aimed to study the B lymphocyte cells subset (naive and memory cells) counts in a cohort of diagnosed CGD patients using flow cytometry.
The present study was conducted on 50 pediatric participants selected from Alexandria University Children’s hospital divided into groups : 25 CGD patients (group I) and 25 healthy age and sex matched control (group II).
Complete blood count (CBC) as well as dihydrorhodamine (DHR) test were done for CGD patients (group I) and controls (group II) then naïve B (IgD +ve & CD27 –ve) and memory B (CD27 and CD19+ve) cell counts were analyzed by flow cytometry using the following panel of monoclonal antibodies: IgD, CD27 and CD19.Also when feasible, evaluation of NADPH oxidase protein expression in CGD patients was performed using flow cytometry.