الفهرس 
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Abstract
Vincristine Is A Frequently Used Antineoplastic Drug For Treating Various Cancers. However, Its Induction Of Painful Neuropathy Has Limited Its Use. Developing Curative Preventative And Therapeutic Options For Vincristine-Induced Painful Neuropathy (VIPN) Requires A Better Understanding Of Its Etiology And Pathophysiology. According To Previous Studies, VIPN May Be Due To A Complex Mechanism Of Neuroinflammation, Oxidative Stress, And Nerve Degeneration.
Curcumin Has Anti-Inflammatory And Immunomodulatory Effects, And It Was Used In The Current Study As A Nano Emulsion Formulation To Determine Its Potential Protective Mechanisms Against Vincristine-Induced Neurotoxicity. These Effects Have Been Compared To The Standard Gabaergic Drug (Pregabalin).
Rats Were Randomly Divided Into Six Groups, Including; Normal Control (0.5 Ml PBS, P.O, For 14 Days), Vincristine (150 µg/Kg, I.P, Once Every Two Days, For Ten Days), Vincristine + Pregabalin (30 Mg/Kg ̸ Day, P.O For 14 Days), Vincristine + Curcumin (30 Mg/Kg ̸ Day, P.O, For 14 Days), Vincristine + Curcumin Nano Emulsion (30 Mg/Kg ̸ Day, P.O, For 14 Days), And Vincristine + Vehicle [Placebo Nano Emulsion (0.5 Ml, P.O, For 14 Days)].
Assessment Of Pain Behaviors Was Carried Out By Testing Thermal Hyperalgesia And Cold Allodynia Via Hot Plate Test And Tail Immersion Test, Respectively, On Days 0, 7, 10, And 14. On The 14th Day, Rats Were Sacrificed, And Sciatic Nerve And Spinal Cord Tissues Were Collected And Used To Estimate Catalase (CAT), Superoxide Dismutase (SOD), Interleukin-10 (IL-10), And Peroxisome Proliferator-Activated Receptor Gamma (PPAR-Γ), Respectively. Sections Of The Nerve Were Used For Histopathological Examination And Immunostaining For S100 Protein, Nuclear Factor Kappa B (NF-Κb), Inducible Nitric Oxide Synthase (Inos), And Heat Shock Protein 70 (HSP70).
The Results Indicated That Curcumin In The Nano Emulsion Form Significantly Decreased Thermal Hyperalgesia And Cold Allodynia. Moreover, Curcumin Significantly Increased Sciatic Nerve Levels Of CAT, SOD, And IL-10 And Spinal Cord Levels Of PPAR-Γ. Immunostaining Revealed A Significant Decrease In Nerve NF-Κb And Inos Expressions And A Significant Increase In S100 And HSP70 Expressions In The Sciatic Nerve Sections from The Animals Treated With Curcumin Nano Emulsion Compared To Vincristine Group. Moreover, It Revealed A Normal Histological Structure Of Endoneurium With Myelinated Axon Nerve Fibers.
In Conclusion, These Results Suggested That Curcumin In The Nano Form Was Able To Alleviate The Painful Neuropathy Induced By Vincristine. That Could Be Attributed To The Upregulation Of PPAR-Γ, HSP70, S100, And IL-10, Indicating That These Modulators Could Be Crucial Regulators For Managing Chemotherapy-Induced Neuropathic Pain. Moreover, The Enhancement Of Curcumin’s Bioavailability Markedly Intensified Its Therapeutic Effects.