الفهرس 
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Abstract
Abstract
This study was carried out on 156 randomly selected bovine calves for parasitological, molecular, phylogenic identification and statistical analysis to study calf cryptosporidiosis during the period from august 2021 to Aril 2023. We also studied some of the risk factors that affect the spread of Cryptosporidium infection. Parasitological examination of 156 fecal samples revealed that (100/156, 64.1%) were positive for Cryptosporidium. The prevalence of cryptosporidium in fecal samples collected in different seasons showed a very high significant during the summer, amounting to 14.28 times the prevalence in the winter. The results revealed that the increase in infections was not significant in different age groups. The infection rate was significantly higher in calves of American origin (48/58, 82.7%) than in calves of German origin (52/98, 53%). Regarding the effect of fecal consistency on the total prevalence of Cryptosporidiosis in calves, there was a non-significant increase in calves suffering from watery diarrhea (85/85, 100%), and in mucous diarrhea (15/15, 100%), and the infection rate was non-existent in calves with normal fecal samples. (56/0, 0.0%). Four samples positive for Cryptosporidium by microscopic examination were molecularly examined using PCR, and all samples were positive. Genetic sequencing was done for a sample of them, and this sequence was registered in the International Gene Bank under the number LC770056, and phylogenetic and genetic analysis was done, which proved that Cryptosporidium has great genetic similarity to other strains discovered from other animals in addition to humans in Egypt and different parts of the world.
Keywords: cryptosporidium; Infectious; Economic; calves; PCR; Phylogenetic analysis