الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
69)Dental abscess is a widespread, persistent, and non-communicable disease worldwide. Bacterial infection is one of the most important factors causing tooth decay. This study aimed to isolate and characterize bacteriophages, as a biocontrol agent, against certain antibiotic-resistant bacteria causing dental abscess. Here, five strains of dental abscess -causing bacteria E. faecalis were isolated and characterized biochemically using the automated Vitek 2 system. Antibiotic sensitivity pattern of the isolated bacteria was assessed against selection of antibiotics. The isolates showed resistance against most of the tested antibiotics.
To overcome this problem, two lytic phages E. faecalis_phage-01, E. faecalis_phage-02 were isolated, identified, and applied to control the growth of E. faecalis_phage-01 E. faecalis_phage-02. Phages were identified morphologically using TEM and showed that faecalis phage-01 is related to Siphoviridae and E. faecalis_phage-02 belongs to Myoviridiae The two phages exhibited high lytic activity, high stability, and a narrow host range. The one-step growth curve of phages showed burst sizes of 86.75 and 115.76 PFU/cell with latent periods of 25 and 30 min for E. faecalis_phage-01, E. faecalis_phage-02 respectively.
Hence, this study indicates that the isolated bacteriophages are promising biocontrol agents that could challenge antibiotic-resistant dental caries bacteria to announce new successful alternatives to antibiotics.
Two E. faecalis phages were detected in environments with temperatures between 10 and 60 °C. However, following 60 min of heating at 80°C, no live phages were found. After 60 min at 37°C, it was discovered that two E. faecalis phages were resistant to a pH range of 4-11. The use of phages as biocontrol agents in dental procedures is made easier by phage stability under demanding environmental conditions. At temperatures between 10 and 60°C, and pH range between pH 4 and 11 E. faecalis phage-01 and E. faecalis_phage-02 were stable. E. faecalis_phage-01 and E. faecalis_phage-02 have no cytotoxic effect on oral epithelial cell lines tested in vitro.