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Abstract The present study was aimed to establish a reference range for normal body temperature in dogs. To achieve this goal, two groups of animals were examined: Eleven of indoor native breed puppies were studied over a six-months period, during which their temperatures were monitored using various methods. These animals were examined at three different time points (at 2, 4 and 6 months of age). At each time point different body temperature were recorded for seven consecutive days and CBC were evaluated once at each age point. Forty-seven client-owned dogs of different breed, age, and sex, were referred for a general health check, underwent temperature measurements using three different methods, and their temperatures were recorded. CBC was also performed to confirm the health status of dogs. Environmental temperature was also recorded in both experiments. Temperature in both experiments was recorded by the same devices. Rectal temperature was recorded using a mercury thermometer and a digital thermometer. Axillary temperature was measured by digital thermometer while tympanic membrane temperature recorded by aural thermometer specifically designed for dogs. Results summary: • Temperature by different method used in the study at 6 months age showed a significant decrease compared to that at 2 and 4 months of age. • Sex and breed did not significantly effect on body temperatures. • There was no significant difference between rectal temperatures measured by mercury and digital thermometers. • Axillary temperature and tympanic membrane temperature underestimate rectal temperature. • Environmental temperature was not significantly correlated with rectal temperature, axillary temperature and tympanic membrane temperature. • The present study revealed that the reference interval for rectal temperature measured by both mercury thermometer and digital thermometer were higher than previously established ones. |