الفهرس | Only 14 pages are availabe for public view |
Abstract This study aimed to investigate the antidiabetic activity of Matricaria aurea (Golden Chamomile, MA) in a type 2 diabetic rat model, then elucidate the action mechanisms by using methods from molecular biology, bioinformatics, and network pharmacology. Our results demonstrated that only the polar ethanolic extract of MA had remarkable antidiabetic activity, while less polar extracts did not show any promising activity. Furthermore, it improved dyslipidemia, insulin resistance status, ALT, and AST levels. LC-MS/MS analysis of MA ethanolic extract identified 62 compounds, including the popular chamomile flavonoids apigenin and luteolin, other flavonoids and their glycosides, coumarin derivatives, and phenolic acids. Bioinformatics and network pharmacology analysis revealed that 46 phytochemicals in MA can modulate many proteins associated with inflammatory pathways, oxidative stress pathways, and energy-sensing/endocrine/metabolic pathways. q- RTPCR gene expression analysis showed that MA ethanolic extract was able to significantly upregulate PIK3R1 and downregulate IL1B, PCK1, and MIR29A. Moreover, the activity of the antioxidant hub enzymes was substantially increased. Molecular docking scores were also consistent with Author Yassin Ismail Attia Abdelhamid Title Evaluation of the Antidiabetic Effect of Matricaria aurea (loefl.) in Streptozotocin-Induced Diabetic Rats Faculty Faculty Of Pharmacy, Suez Canal university Department Department of Biochemistry Location Ismailia, Egypt Degree M.Sc. in Pharmaceutical Sciences (Biochemistry) Date 25/7/2023 Language English Supervision committee Prof. Dina Mohamed Abo-Elmatty, Associate Prof. Dina Mostafa Fahmy, and Dr. Samy Mohamed Saleh the networks’ predictions. Based on experimental and computational analysis, this study revealed for the first time that MA exerted antidiabetic action via simultaneous modulation of multiple targets and pathways, including inflammatory pathways, oxidative stress pathways, and energysensing/ endocrine/metabolic pathways. Key words: Matricaria aurea (MA), Diabetes, Network pharmacology, LCMS/ MS, metabolic pathways, Oxidative stress, inflammatory pathways. |