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Abstract The focus will be on the extraction, isolation, and structural elucidation of secondary metabolites obtained from various natural sources. One of the most important bee products is royal jelly, which is highly valued for its richness in proteins. One such biologically active protein isolated from royal jelly is royalisin, composed of 51 amino acids. In another study, our aim is to synthesize royalisin using the solid-phase peptide synthesis (SPPS) method. We concentrate on the chemical examination of specific fatty peptides in B. subtilis. Here, the GNPS approach, along with LC-MS/MS, was utilized as a powerful technique to identify the structure of fatty peptides from these bacteria. Additionally, we aim for comprehensive plant chemical profiling alongside targeted isolation by assessing antimicrobial activities of novel metabolites from the known gum arabic. Chemical investigation of AG led to the identification of seven new compounds. The structures of these compounds were elucidated using MS and NMR, along with comprehensive analysis and comparison of two types of Lantana genus using MS concerning their cytotoxic and antioxidant activities. Targeted MS profiling of methanol extracts also led to the preliminary identification of 59 compounds. |