الفهرس 
Review of literatureOnly 14 pages are availabe for public view
 |  | from | 157 |  |  |
| | | from | 157 | | |
Abstract
The purpose of this study is to investigate the effect of intra-canal treatment modalities in patients with periapical lesions on the local and systemic levels of specific inflammatory mediators.
Sixty medically free patients of both genders were selected. The patients were undergoing clinical and radiographic examination to confirm the existence of necrotic pulp with periapical lesion.
According to intracanal medicament that were used between visits, patients were allocated into the following groups:
group I: (n=15) (control) Conventional root canal treatment or retreatment were done. Root canals were medicated with conventional calcium hydroxide.
group II: (n=15) Conventional root canal treatment or retreatment were done. Root canals were medicated with triple antibiotic paste.
group III: (n=15) Conventional root canal treatment or retreatment were done. Root canals will be disinfected using LASER beam.
group IV: (n=15) Conventional root canal treatment or retreatment were done. Root canals were left empty between visits.
116
summary
Systemic and local samples were taken to measure local and systemic cytokines (IL1β, TNF α) before and after treatment using ELISA assay. The result showed that the systemic levels of IL-1β and TNF-α before and after treatment, it is evident that all groups exhibited varying degrees of change. Notably, group IV showed a substantial increase in both IL-1β and TNF-α levels post-treatment, indicating a heightened systemic inflammatory response.
Conversely, Groups I, II, and III demonstrated more modest changes in cytokine levels, suggesting a comparatively lesser systemic inflammatory reaction following treatment.
local levels of IL-1β and TNF-α in intercrevicular fluid showed differential responses to treatment across the groups. While all groups exhibited reductions in cytokine levels post-treatment, the magnitude of decrease varied. group II, treated with triple antibiotic paste, demonstrated the most significant reduction in both IL-1β and TNF-α levels locally.
Triple antibiotic paste contains combinations of antibiotics (metronidazole, ciprofloxacin, and minocycline) with broad- spectrum antimicrobial activity. This combination effectively targets a wide range of endodontic pathogens,
117
summary
including facultative anaerobes, obligate anaerobes, and Enterococcus faecalis, which are often associated with persistent periapical infections.