الفهرس | Only 14 pages are availabe for public view |
Abstract Vitiligo is an acquired pigmentation disorder characterized by the loss of epidermal melanocytes and/or their function. It is a relatively common disorder, with a prevalence rate of 0.2–1.8%. Although the exact etiology of vitiligo remains elusive, autoimmunity is believed to play an important role in disease pathogenesis, as vitiligo is often associated with autoimmune diseases. Noncoding Ribonucleic Acid (RNAs), including lncRNA, miRNA and circular RNA , play an important role in the regulation of gene expression. Studies of noncoding RNA in the etiology of vitiligo have focused primarily on miRNAs. MicroRNAs are evolutionarily conserved non-coding RNA molecules (22 nucleotides long, single-stranded) which regulate gene expression through imperfect base coupling with complementary sequences in their target mRNA, causing its translational suppression or degradation. There are elatively few data reported on the epigenetics of vitiligo, and in particular on the role of miRNAs MiRNAs are a class of noncoding single-stranded RNA with a length of approximately 20–24 nucleotides. During miRNA processing in the nucleus, pri-miRNAs are first synthesized by RNA polymerase and then modified into pre-miRNAs. Pre-miRNAs are transported out of the nucleus. Studies have shown that approximately 60% of human encoded MiR-21-5p can be considered an important regulator of the melanogenetic process. MiRNAs are short single-stranded non-coding RNAs that regulate degradation and expression of mRNA and play a wide range of key roles in different biological processes. They mediate different stages of melanocyte differentiation, growth, and apoptosis through a variety of pathways and can mediate melanin production by targeting key enzymes protein genes are regulated by miRNAs. The primary objective of this study was to determine whether Micro RNA-21-5p could serve as a diagnostic or prognostic marker in vitiligo. This involved assessing the relative expression levels of Micro RNA-21-5p among individuals with vitiligo compared to a healthy control group . This study was carried out on 25 patients presented with different clinical varieties of vitiligo and 25 age and sex matched apparently healthy individuals as a control group, Blood samples were collected, and the expression levels of Micro RNA-21-5p were measured using quantitative Real Time Polymerase Chain Reaction (RT-PCR). |