الفهرس 
Introduction & Aim of the workOnly 14 pages are availabe for public view
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Abstract
Background: Ischaemic heart disease is a major cause of death allover the world. Understanding the molecular basis of the disease is of great significance for its early detection and treatment Aim of the work: The aim of the present study is to evaluate the usage of proteomics as prospective markers in early diagnosis of IHD. Method: The present study included 50 subjects with age ranged from 40 to 65 years. They were divided into 5 groups; AMI, unstable angina, stable angina, old MI and control groups. Serum was collected, divided into aliquots and stored frozen at 80C until analyzed for proteomics, MDA, total antioxidants, nitrite and total proteins. Serum MDA, nitrite and total proteins were estimated by colorimetric assay, while serum total antioxidants were estimated by colorimetric inhibition assay with fixed time point. Regarding proteomic study, 2DE was applied to separate the set of the serum proteins. Results: There is significant decrease in the number of protein spots in the fingerprints of all IHD groups (AMI, unstable angina, stable angina and old MI) when compared with control group. Protein spots with low molecular weight (< 45 kDa) were markedly decreased in both AMI and unstable angina fingerprints when compared with control group, while in both stable angina and old MI fingerprints they were completely lost. Serum MDA level shows significant increase in AMI & unstable angina groups and insignificant increase in stable angina & old MI groups when compared with control group. There is significant decrease in serum total antioxidants and nitric oxide in all IHD groups when compared with control group. There is insignificant correlation between the number of protein spots and serum MDA, nitrite and total antioxidants in all groups. Except in stable angina, a significant negative correlation was found between the number of spots and serum MDA only. Conclusions: The comparative proteome analysis is a method with power to develop important insights into economic and noninvasive diagnostic markers in IHD. Also, the intensification of lipid peroxidation process and the impairment of antioxidant activity together with reduced nitric oxide bioactivity confirm the presence of oxidative stress in IHD.