الفهرس 
Introduction and aim of the work
Brief survey of clinical virologyOnly 14 pages are availabe for public view
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Abstract
Traditional methods of diagnosis often involve complex and e-consuming procedures. Recently, attempts have been made to velop rapid means for virus diagnosis, as this might help in rly institution of appropriate therapy and prompt control of read of viruses. In order to promote more rapid laboratory diagnosis of rus infection, this study was performed on two particular ruses, influenza virus and cytomegalovirus. The study on influenza virus was undertaken in two parts. In The first part vairity of clinical samples was collected from 45 patients with acute respiratory tract infection, 9 male and 26 female, with age range from 1 month to 75 years. The samples were 29 throat swabs, 12 traqheal aspirates, Putum samples and2 bronchial washings. In addition3 ost-mortem biopsies(2 lung and 1 cardiac) and 3 laboratory nfluenza A isolates were examined. For virus isolation, tubes with monolayers of primary rhesus monkey kidney cells (MKC) and Vero cells were used. The growing virus was detected by haemadsorption (H-ad) and identified by haemadsorption inhibition. For The direct fluorescent test (DFT), the fluorescein-conjuga ted monoclonal antibody was applied for detection of influenza virus, 24 hours post-inoculation on centrifuged vials that contained coverslips with MKC. For electron microscopy (EM), specimens were stained with A Potassium phosphotungstate (PTA) and then examined by EM. The results show that, MKC gave positive haemadsorption in 5151 (9.8%), and Vero was positive in onl~ 2151 (3.9%). A highly significant difference is noted (P < 0.001, normal difference test). Positive results were ob ta in ed in3151 (5.9%) in DFT, in comparison with 5151(9.81o) and 2151 (319%) on MKC and Vero cells respectively. DFT seems to be of comparable sensitivity with AIKC(315; 60%), but detected one more positive than Vero cells In addition DFT gave results more rapidly,24 hours.