الفهرس 
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Abstract
Oxidative stress is an important process that occurs in vivo and is considered one of the main causes of molecular damage to the cellular and the tissue structures. These changes can accumulate in the biological structures during ageing or even to be causes of the ageing process. The aim of this work is to investigate the levels of oxidative stress markers as lipid peroxidation products (malondialdehyde), nitrite (nitric oxide), total antioxidant status, total thiols group and metal binding proteins in relation to ageing. This study was conducted on 50 healthy non smoker volunteers with age range 20-75 years. It was found that oxidative stress is higher in young individuals and this supports that the free radical mediated damage is a cause, not an association, of the gradual ageing process. Also, the decline of the nitrite in elderly was found to be a resultant factor from the ageing associated damage of the endothelium. The total antioxidant status did not change with ageing because it may be affected by several factors and so the assessment of individual antioxidants may be of better value. Ageing was associated with a progressive protein damage apparent from the decreased levels of the total thiols with ageing as well as decreased albumin levels. The metal binding protein; ferritin and uric acid are not changed with ageing and this supports that the oxidative stress is not higher in older subjects. Oxidative stress is an important process that occurs in vivo and is considered one of the main causes of molecular damage to the cellular and the tissue structures. These changes can accumulate in the biological structures during ageing or even to be causes of the ageing process. The aim of this work is to investigate the levels of oxidative stress markers as lipid peroxidation products (malondialdehyde), nitrite (nitric oxide), total antioxidant status, total thiols group and metal binding proteins in relation to ageing. This study was conducted on 50 healthy non smoker volunteers with age range 20-75 years. It was found that oxidative stress is higher in young individuals and this supports that the free radical mediated damage is a cause, not an association, of the gradual ageing process. Also, the decline of the nitrite in elderly was found to be a resultant factor from the ageing associated damage of the endothelium. The total antioxidant status did not change with ageing because it may be affected by several factors and so the assessment of individual antioxidants may be of better value. Ageing was associated with a progressive protein damage apparent from the decreased levels of the total thiols with ageing as well as decreased albumin levels. The metal binding protein; ferritin and uric acid are not changed with ageing and this supports that the oxidative stress is not higher in older subjects. On this basis, the free radical theory of ageing may explain some particular events occurring with ageing but still the ageing is not a single process. Thus, additional evidence is needed to clearly define the nature and the exact mechanism of ageing. Therefore, the assessment of oxidative process on a cellular basis is recommended in future studies.