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Abstract Three hundred random samples (100 of raw milk, 50 each of Damitta cheese, kareish cheese, ice cream and plain yoghurt) collected from different localities of ElDakahlia province, Egypt, were examined for the presence of verocytotoxigenic E. coli especially E. coli O157:H7. The incidence of VTEC organisms by using direct plating method isolated from raw milk samples were (1%), ice cream (2%), yoghurt (18%), kareish cheese (36%) and Damitta cheese (10%). The competitor species could be identified as Klebsiella, Proteus, Pseudomonas and Plesiomonas and were isolated from raw milk and dairy products in different percentages. The incidence of VTEC isolated from raw milk and other milk products by using the enrichment method were 16% from raw milk, 4% from ice cream, 8% from yoghurt, 42% from kareish cheese and 8% from Damitta cheese. The incidence of VTEC O157:H7 detected by direct plating and enrichment methods using CTSMAC medium and Latex agglutination test was (18%, 12%) from kareish cheese; (0%, 4%) raw milk; (2%, 2%) ice cream; (2%, 2%) yoghurt; and (2%, 4%) Damitta cheese, respectively. It was concluded that verotoxigenic producing E. coli O157 and other verotoxigenic E. coli survived acidity percent in plain yoghurt and sodium chloride concentration in kareish and Damitta cheese. Regulations to prevent contamination of milk and dairy products with VTEC especially O157:H7 and to safeguard consumers from infections were discussed. |