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Abstract Aflatoxins are a group of mycotoxins secreted by number of molds from penicillium and Aspergillus but the most molds producing aflatoxin are A. flavus and A. parasiticus. Aflatoxin B1 is considered the most harmful and toxic aflatoxins so it has got great attention to study its effects. The obtained results could be summarized as follows: A Survey for fungi and aflatoxin B1 in poultry feedstuffs in Dakahlia and Damietta Governorates. Isolation and identification of nine species of fungi existed on poultry feedstuff samples on PDA + streptomycin media. The highest percentages of fungi infection were observed in regions of ElSerw (88.8 %), Sherbeen (77.7 %) and Mansoura (70.58 %). The lowest infection one was observed in Shawa region (54.54 %). Out of 103 various feedstuff samples (representing 9 sources from 9 locations) 34.95 % were AFB1positive with a concentration ranged from 0.8 to 261 ppb. The highest contamination level was found in yellow corn samples (55.5 %) with a concentration ranged from 41 to 211.9 ppb. While poultry feeds (29.41 %) were AFB1positive with a concentration ranged from 14 to 261 ppb. About 50 % of feedstuff samples must be rejected for their higher contamination levels than the permissible levels in most world countries (50 ppb). The contamination level was higher too in Sherbeen and Damietta regions (55.5 and 50 %) than the other regions with a concentration ranged from 5.2 to 124.8 and from 0.9 to 73 ppb, respectively. B Biological control of aflatoxin B1 production from A. parasiticus using medicinal and aromatic plants under investigation: Phytochemical screening of the obtained hydro alcoholic extracts was carried out to detect flavonoids, alkaloids, saponins, tannins, Terpenes, and glycosides. Concerning the effect of the different plant extracts on growth diameter (Cm) and growth weight (g) of A. parasiticus. Results revealed that all solvent extracts have significant inhibitory effect on growth diameter (Cm) of the tested fungi. The most efficient one was pet. ether of Thyme, Damsisa and Halfa bar. However, Chamomile chloroform extract was the most effective one. from the previous results, it could be concluded that the most efficient plant was Halfa bar that cause a decrease value of 71.4 % for growth diameter (cm), 58.33 % for growth weight (g) and 50.57 % for aflatoxin production. In conclusion, Halfa bar, Damsisa, Thyme and Chamomile caused a remarkable inhibition against A. parasiticus growth and aflatoxin production. These plants could be used in controlling aflatoxin formation in feedstuffs and food without any harmfull effects on human or anima. |