الفهرس 
REVIEW OF LITERATUREOnly 14 pages are availabe for public view
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Abstract
This study was undertaken to evaluate the effect of addition of some antioxidants (ascorbic acid and glutathione) to two types of extenders, Egg-Yolk Tris (EYTE) and Egg Yolk-Citrate (EYCE) on sperm characteristics in semen stored at different temperature degrees. Semen was collected from 10 buffalo bulls twice a week via an artificial vagina. Ejaculates with mass motility >70% were pooled and divided into seven parts for each extender supplemented with 0, 0.5, 1.0 and 1.5 g/l of ascorbic acid (AA) and 0.4, 0.8 and 1.2 mM of glutathione (GSH). Sperm characteristics including, motility, livability, abnormality, intact acrosome and curling of spermatozoa were evaluated in semen stored at room temperature for 0, 24, 48, 72 and 96 hours; at cooling temperature (5oC) for 0, 24, 48, 72 and 96 hours and at different semen processes for freezing (in post-diluted, post-equilibrated and post-thawed semen.
The obtained results could be summarized at the following:
1. Buffalo semen could be preserved for 4 hours at room temperature with acceptable percentage of sperm motility (60%), livability (63%), intact acrosome (65%) and abnormality (13.4%), when it was diluted by EYCE supplemented with 0.4 mM of enzymic antioxidant (GSH) at a dilution rate of 1:10.
2. Using EYTE supplemented with natural antioxidant (AA) at a level of 0.5 g/l or enzymic antioxidant (GSH) at a level of 0.4 mM in semen dilution at a rate of (1:10) resulted in bull spermatozoa to maintain their fertility capacity at cooling temperature (5oC) for 72 hours.
3. Addition of 0.4 mM of GSH as an antioxidant to EYTE during cryopreservation of buffalo semen maintained viability, livability, morphology, and cell membrane integrity during freezing processes.