الفهرس 
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Abstract
Introduction: Canola (Brassica napus L.) was developed from rapeseed by Canadian plant breeder for its superior nutritional qualities. It’s name is obtained as a short of Canadian Oil Low Acid. Canola contains over than 40% oil in their seeds and the meal residue after oil extraction contain 36% to 44% protein. The oil is low in saturated fat, a good source of omega three and rich in vitamin E. Thus, the improvements of canola for their yield components and oil percentage are considered one of the major aim of canola breeders.
•Aim of the study: Evaluate the DH lines in addition to their origin in order to detect the gametoclonal variation. The second aim is to evaluate the performance these DH lines as a potential parents of hybrids in order to detect the genetical variation among these genotypes for both in vitro and field traits
•Material and methods: During two successive seasons 2005/ 2006 and 2006 / 2007, the four DH lines (DH1, DH2, DH3 and DH4) in addition to the origin of these DH lines were evaluated in a randomized complete blocks design with three replications under field condition and with four replications for in vitro traits. At the season 2006 / 2007, the four double haploids lines and their 12 F1 hybrids were arranged in a randomized complete blocks design with three replications.
•Results: The DH lines are varied in most of their agronomic traits, but they are similar in their response to anther culture. Exception DH3, no significant difference was observed between DH lines compared to their origin in most of studied agronomic traits• Recommendations: The proper breeding program for improving these traits is hybridization among doubled haploid lines, especially which involved DH3 line as one of their parents to utilize the desirable gametoclone resulted from anther culture technique