الفهرس 
Oocyte recovery
Functions of follicular fluidOnly 14 pages are availabe for public view
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Abstract
This study was carried out to investigate the: (1) Effect of different periods 5, 6, 7, 9, 12 and 24 hours of ovary storage at 25 °C on recovery rate and oocyte categories of dromedary camel oocytes. (2) Effect of using of follicular fluid alone or in combination with fetal calf serum from dromedary camel on oocyte maturation in vitro. (3) Effect of storage period of camel testes at room or refrigerator temperature after slaughter on recovery rate and characteristics of epididymal spermatozoa. (4) Effect of antioxidant supplementation to the extender on characteristics of epididymal spermatozoa stored at room and refrigerator temperature. (5) Possibility of in vitro producing camel embryo from oocytes and spermatozoa recovered from slaughtered animals. The present study could conclude that: (1) The storage of dromedary camel ovaries at 25°C for 5h was effective for maintaining the oocytes quality and recovery rate compared with the other storage period up to 24hrs. (2) Supplementation of maturation medium with 10% fetal calf serum significantly (P<0.001) showed the highest frequency distribution of oocytes (59.5%) at metaphase II. (3) Testes of camel can be stored at (5 °C) for 24 hrs without any effect on the characteristics of epididymal spermatozoa. (4) Extended semen with tris extender supplemented with 0.4 mM of GSH at (5 °C) showed improve of epididymal spermatozoa. (5) The results of in vitro development competence of dromedary camel oocyte indicated that satisfied success rate of cleavage of in vitro matured camel oocyte using epididymal spermatozoa.