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العنوان
Physiological characteristics of enterobacter sakazakii isolated from milk products /
المؤلف
Abo Ziada, Dina Mohamed El-Sayed Ali.
هيئة الاعداد
باحث / دينا محمد السيد علي أبو زيادة
مشرف / محمد محمد إبراهيم زين الدين
مشرف / وليد محمود الشارود
الموضوع
Enterobacter sakazakii. Milk Products and Druggan. Forsythe. Iversen (DFI) agar.
تاريخ النشر
2009.
عدد الصفحات
99 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
العلوم الزراعية والبيولوجية
تاريخ الإجازة
01/01/2009
مكان الإجازة
جامعة المنصورة - كلية الزراعة - Dairy Department
الفهرس
Only 14 pages are availabe for public view

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Abstract

This study was conducted to examine the physiological characteristics and antibiotic resistance of an emergent pathogen, Enterobacter sakazakii, isolated from milk and dairy products distributed and/or produced in Egypt. During the course of this study, 235 samples of raw milk, dried milk and some local dairy products were collected from different areas representing eight Egyptian governorates. Eleven suspect isolates of Ent. sakazaki were recovered from a total of 235 examined samples of milk and dairy products. Following morphological and biochemical examinations using the Api 20E identification system, 6 out of the 11 suspect isolates were confirmed as belonging to Ent. sakazakii. Confirmed 6 Ent. sakazaki isolates were recovered from dried milk (2 isolates), stored Domiati cheese (2 isolates) and fresh Domiati cheese (2 isolates). Other examined samples including those of raw milk, Ras cheese, Kariesh cheese, and yoghurt were free of the pathogen. These results confirmed the association of Ent. sakazaki with dried milk and certain dairy products such as Domiati cheese. This reflected the ability of this pathogen to tolerate dry and salt stress in dried milk and Domiati cheese, respectively. The absence of the pathogen in fermented dairy products including yoghurt, Ras cheese and Kariesh cheese may indicate its sensitivity to acidity. The biochemical characteristics of the confirmed 6 Ent. sakazakii isolates were examined using the Api 20E miniaturized identification system. The six isolates of Ent. sakazakii were all susceptible to 11 antibiotics. These results show that it is possible to eradicate Ent. sakazakii using currently available generations of antibiotics, yet it will still be important to develop measures to avoid the transmission of this pathogen via foods rather than relaying on antibiotics to treat its infections.