الفهرس 
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Abstract
Plant tissue culture techniques attempted at the early days of interest in this field. Lagged behind animal and human tissue culture techniques due to the late discovery of plant hormones. Most work in the field was carried out on certain parts of the plants or at least whole tissue. These parts after selected have to be isolated, disinfected and transferred to a nutrient medium under aseptic conditions. The cells picked from the plant parts are allowed to grow and the callus pieces are transferred to fresh medium and used as inocula. Through the manipulation of cultural conditions. The cells are induced to produce secondary metabolites and products of chemical and pharmacueutiical importance are produced by different plant tissues.
In present work catharanthus (L) G. Don, widely known as Madagascar periwinkle, was piched to obtain calli for the production of chemically and pharmaceutically important products. Basically, these are a group of compounds closely related in some instances and will be referred to as indole alkaloids. Basically a complicated medium, called Murashige and skoog medium was used in the present work and with some alerations in some experiments.
The production of the potent indole alkaloids by cell cultures can be regarded as differentiation process governed by both environmental conditions and the genotype of the material used.