الفهرس 
Introduction and aim of the workOnly 14 pages are availabe for public view
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Abstract
In the present investigation, accurate diagnosis of Gram negative bacteria which is clinically isolated from urine specimens from different urinary tract infections (UTIs) was carried out. By using biochemical identification methods, all isolates could be identified to the genus level only and some isolates could be identified to the species level. Antimicrobial susceptibility testing showed that imipenem could be considered as the drug of choice for treatment of UTIs caused by multi-resistant isolates. By analysis of total cell protein using sodium dodecyl sulfate polyacrylamide gel electrophorosis (SDS-PAGE) technique the isolates could be classified into different patterns. By using molecular diagnosis methods, all isolates could be identified to the genus and species level, where the genomic DNA extracted from some isolates and by using polymerase chain reaction different regions of 16S-rRNA gene were amplified and sequenced according to Sanger sequencing method and by comparison of the obtained sequences with standard sequences in the gene bank all isolates could be identified to the genus and species level. In the present study, also the relatedness between different isolates was determined by construction of phylogenetic trees using Megablast software.