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Abstract The present study aimed to verify genetic purity of three wheat varieties namely Gemmiza 10, Giza 168 and Sakha 94 using morphological, biochemical characters as well as grain yield of each variety. Two field experiments were conducted at the Experimental Farm of Tag El-Ezz. Agricultural Research Station Dakahlia Governorate, Agricultural Research Center (A.R.C.), Egypt during the two wheat successive seasons of 2007/08 and 2008/09 were conducted. The results indicated that the Sakha 94 variety was identified by absent anthocyanin coloration of coleoptile.Absent flag leaf area anthocyanin coloration, strong culum glucosity.Very long plant height and medium coloration with phenol test 1 %. The Giza 168 variety was identified by medium anthocyanin coloration of coleoptil.Medium of flag leaf area anthocyanin.Very light with phenol coloration.Gemmiza 10 variety identified by strong anthocyanin coloration of coleoptil.Very strong of flag leaf area anthocyanin.Short plant height, very dark with phenol coloration. Results indicated that the percentage of off-types increased as the farmer relied on himself for preparing seeds for crop production. The grain yield and genetic purity based on number of off-types and seed fingerprint were significantly reduced from planting farmer-saved seeds compared with basic, registered and certified seeds. It is possible to utilize farmer- saved seed for one year of certified seed provided that the farmer takes out the off-types from the part of his field he keeps for next planting. Laboratory testing including phenol test of the seed came the same conclusion. Conclusion It could be summarized that the sowing on 20th Nov gave the best seed quality by using Gemmiza 10 wheat variety also certified seed has been produced under a seed certification program is the best for high seed yield due their high vigor, viability and genetic purity. |