الفهرس 
Symptoms of breast cancerOnly 14 pages are availabe for public view
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Abstract
Breast cancer is one of the most dreaded diseases of women. Breast cancer represents a major psychological, social and economic burden on the Egyptian community. Late diagnosis of breast cancer is associated with poorer prognosis and higher morbidity and mortality rates. Tumor markers are potentially useful in screening cancer. However, lack of sensitivity combined with a lack of specificity precludes the use of them for the early diagnosis. Thus, new markers are needed. Therefore, the aim of the present study was to identify, purify and quantify Ck1 from blood of breast cancer women aiming to using it in diagnostic potential. Serum samples from 75 breast cancer female individuals and 27 healthy women were used. The target cytokeratin 1 antigen was purified from sera of breast cancer patients using electroelution technique. The purified cytokeratin 1 was characterized to be protein with molecular weight 68 kDa. Cytokeratin 1 was quantified in serum samples of 75 breast cancer patients using ELISA. There was a highly significant difference (P < 0.001) between the average cytokeratin 1 level in serum samples of 75 breast cancer women ”6.4 ± 7.1 µg/ml” compared with ”0.04 ± 0.05 µg/ml’’ as an average serum level of 27 controls individuals. The sensitivity, specificity for the cytokeratin 1 antigen gave the values 77.3 %, 100%, respectively. The positive predictive value, negative predictive value and efficiency were calculated 100%, 61.3% and 83.3%, respectively. The area under the ROC curve of cytokeratin 1 antigen was 0.96 which is statistically highly significant (P<0.0001). In conclusion and based on the results, the quantitation and characterization of the purified 68 kDa cytokeratin 1 antigen may be of interest to be included in serodiagnosis of breast cancer.