الفهرس 
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Abstract
Squamous cell carcinoma of the cervix constitutes 80% of cervical malignant tumors.
Most invasive carcinomas are preceded by a pre-invasive phase, either dysphasia, or carcinoma in-situ. Evaluation of tumor aggression and biologic behaviour is sometimes difficult by the usual histopathologic techniques.
Recently, molecular biology has served as a new tool for understanding carcinogenesis and it’s conditional relationship to tumor behaviour and prognosis.
Mutations of supressor gene ?53 result in a mutant protein form which accumlates within malignant cells to be readily detected by immunohistochemical staining procedures.
Detection of such amplified mutational events may have prognostic significance in cervical cancer.
The present study aims at examination of P53 protein overexpression in a series of cervical lesions including : Normal, pre-malignant lesions and infiltrating squamous cell crcinoma, to study the relationship between altered expression of P53 protein and the progression of cervical carcinoma.
This study included 48 cases of normal premalignant and malignant lesions of the cervix uteri. Also, a case of colonic adenocarcinoma was known to be reactive to P^3 was use(^ as a positive control and manifested highly positive reaction.
Three slides each of them is four micron-thick were cut from each paraffin block. Sections were stained with haematoxylin and eosin for histopathologic studies.
The indirect immunoperoxidase technique was
•used for detection of ?53 immunoreactivity. Positive staining was indicated by a brown colouration in the nuclei of atypical cells.
In positive cases, the number of stained nuclei per high power feild as well as the intensity of the reaction was recorded.
As regards our study,normal cervical tissues were negative whether in normal cervix or J normal mucosa beside malignant tissues.