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Abstract
The diagnosis of allergy depends on the use of serological assays to measure specific IgE. We detected specific IgE using low- level analyte detection by chemiluminescence. This is a sensitive quantification method as it fulfils many of the requirements for good evaluation being time saving, precise, with very low detection limits and large linear concentrations that can be measured with excellent regression coefficient.
The study included 40 atopic children aged between 3 years and 15 years with known history of allergic conditions such as allergic rhinitis, asthma, or atopic dermatitis and 18 of them were evaluated by allergy skin testing as well.
Conclusion: Chemiluminescence test is the preferred method to detect specific IgE antibodies in vitro as it has shown to have good reproducibility and to give reliable results. It also showed superiority over skin testing